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With the help of the Laser-Speckle phenomenon different in-vivo diagnostic applications can be performed at the surfaces of different tissues such as the skin, the retinal skin and the cerebral cortex. In this study the Laser light scattering phenomenon is used to measure blood flow and hemoglobin concentration change. There are a lot of different approaches to perform these investigations for example with single-mode or multi-mode fibers. A comparison between the two illumination systems is presented together with an experimental setup. The results show that both of the approaches are feasible under specific conditions. Both fiber illumination systems have their advantages and limitations in fulfilling different tasks. The two approaches are tested and evaluated to propose specific applications for every illumination system. With these results future uses of the proposed methods are encouraged.